Phylogenetic Analysis of Bacteria involved in Different Diseases of Tomato (Solanum lycopersicum L.) Crop in District Charsadda, Khyber Pakhtunkhwa Pakistan

Abstract

Tomatoes are exposed to potential microbial contamination. The objective of the current research was to isolate and identify the causal agent of bacterial canker, bacterial wilt, pith necrosis, leaf tip chlorosis, stem rot, and bacterial leaf spot disease on tomatoes in district fields of Charsadda. We collected 50 random samples from different fields in district Charsadda and were cultured on the nutrient agar media and nutrient broth for bacterial growth. Further culture on selective media and biochemical techniques together with molecular sequence analysis was used to probe the particular microbe. A total of 40 samples showed growth on nutrient agar and nutrient broth media. After the biochemical test, the data was analyzed through ABIS software, and isolates were identified as Ralstonia solanacearum, Clavibacter michiganensis, Xanthomonas vesicatoria, Pectobacterium carotovorum, and Pseudomonas corrugata. The isolates were further analyzed using 16S rRNA gene-based sequence analysis. We found that isolates consist of five main species Ralstonia solanacearum, Clavibacter michiganensis, Xanthomonas vesicatoria, Pectobacterium carotovorum, and Pseudomonas corrugata. It was concluded that different bacteria were involved in different tomato diseases like Clavibacter michiganensis in bacterial canker, Ralstonia solanacearum in bacterial wilt, Xanthomonas vesicatoria in bacterial leaf spot, Pseudomonas corrugata in pith necrosis and leaf tip chlorosis, and Pectobacterium carotovorum in stem rot diseases.